NoteMolecular Cloning of Three Genes Encoding G Protein in the White Root Rot Fungus , Rosentnia necatrix JSBA Alpha Subunits
نویسنده
چکیده
is very diMcult to prevent, has done great damage to comrnercially grown grape vines and apple and pear trees, among others, Unfertunately, despite its importance, little is known about the molecular or cellular biology of this fungus. This study is a part of our attempt te better understand the signal transduction pathways mediating the vegetative incompatibility of Rosellinia. In order to isolate the genes encoding the G protein alpha subunit from R, necatrix W8 strain, originally obtained from Dr. Naoyuki Matsumoto (National Institute of Agro-Environmental Science), Genomic DNA was extracted from lyophilized mycelium of the R. necatrix W8 strain using the CTAB (hex-
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